Functional and structural diversity in GH 62 α‐ L ‐arabinofuranosidases from the thermophilic fungus S cytalidium thermophilum

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Functional and structural diversity in GH62 α-L-arabinofuranosidases from the thermophilic fungus Scytalidium thermophilum

The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation, including three genes, abf62A, abf62B and abf62C, predicted to encode glycoside hydrolase family 62 (GH62) enzymes. Transcriptome analysis showed that only abf62A and abf62C are actively expressed during growth on diverse substrates including st...

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Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum.

RNA helicases are indispensable for all organisms in each domain of life and have implications in numerous cellular processes. The DEAH-box RNA helicase Prp43 is involved in pre-mRNA splicing as well as rRNA maturation. Here, the crystal structure of Chaetomium thermophilum Prp43 at 2.9 Å resolution is revealed. Furthermore, it is demonstrated that Prp43 from C. thermophilum is capable of funct...

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Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum.

Prp28 (pre-mRNA-splicing ATP-dependent RNA helicase 28) is a spliceosomal DEAD-box helicase which is involved in two steps of spliceosome assembly. It is required for the formation of commitment complex 2 in an ATP-independent manner as well as for the formation of the pre-catalytic spliceosome, which in contrast is ATP-dependent. During the latter step, Prp28 is crucial for the integration of ...

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Functional diversity of family 3 β-glucosidases from thermophilic cellulolytic fungus Humicola insolens Y1

The fungus Humicola insolens is one of the most powerful decomposers of crystalline cellulose. However, studies on the β-glucosidases from this fungus remain insufficient, especially on glycosyl hydrolase family 3 enzymes. In the present study, we analyzed the functional diversity of three distant family 3 β-glucosidases from Humicola insolens strain Y1, which belonged to different evolutionary...

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Taxonomy of Scytalidium thermophilum, an important thermophilic fungus in mushroom compost

More than thirty isolates of the thermophilic Torula-Humicola complex, mainly obtained from mushroom compost. were examined and compared with the available type cultures of Torula thermophila, Humicola grisea var. thermoidea and H. insolens. Scytalidium thermophilum is considered to be the correct name for all these isolates and type cultures, but the species is very variable in macroscopical a...

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ژورنال

عنوان ژورنال: Microbial Biotechnology

سال: 2014

ISSN: 1751-7915,1751-7915

DOI: 10.1111/1751-7915.12168